The aim of a recent study was to propagate axillary shoots of Cannabis sativa L. using liquid medium in temporary immersion bioreactors. The effect of immersion frequency (3 or 6 immersions per day), explant type (apical or basal sections), explant number (8, 10, and 16 explants), mineral medium (Murashige and Skoog half-strength nitrates, β-A and β-H, all supplemented with 2-µM metatopoline), sucrose supplementation (2, 0.5, and 0% sucrose), culture duration (4 and 6 weeks), and bioreactor type (RITA ® and Plantform TM) were investigated.
As a result, the researchers propose a protocol for the proliferation of cannabis apical segments in RITA or Plantform TM bioreactors. The explants (8 per RITA and 24 per Plantform TM) are immersed for 1 min, 3 times per day in β-A medium supplemented with 2-µM metatopoline and 0.5% of sucrose and subcultured every 4 weeks. "This is the first study using temporary immersion systems in C. sativa production, and our results provide new opportunities for the mass propagation of this species."
Vidal, Nieves & Rico, Saleta & Garrido Gala, José & Sanchez, Conchi & Ferreiro-Vera, Carlos & Codesido, Veronica. (2022). A Temporary Immersion System to Improve Cannabis sativa Micropropagation. Frontiers in Plant Science. 13. 1. 10.3389/fpls.2022.895971.
To read the complete study, go to www.researchgate.net
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